Figure 1
From: Isolation and characterization of endothelial progenitor cells from Rhesus monkeys
The morphology of EPCs isolated from Rhesus monkeys. A. Selected CD34+ mononuclear cells and unselected mononuclear cells were cultured in M 200, observed on day 6 (6D) or day 9 (9D) after culturing and day 4 after the first passage (P1). Both selected CD34+ and unselected mononuclear cells were cerioid shape on day 6, formed cobblestone-appearance colonies on day 9, and become more spindle shape with the loss of stereognosis after first passage. Bar =200 μm. B. Unselected mononuclear cells were cultured in either M 200 or EGM-2 media, observed on day 5 (5D), day 8 (8D) after culturing and day 1 after the first passage (P1). Cells cultured in M 200 proliferated faster than in EGM-2, but cells in EGM-2 had better stereognosis. Bar =100 μm.