2-D Western blot analysis of normal and mutant axolotl hearts using CH1 monoclonal antibody. a. Stage-36 normal hearts show 4 different CH1-recognizable TM isoforms #1, #2, #3, and #4). b. Mutant heart at stage-36 all show 4 CH1-recognizable TM isoforms as in normal hearts at much lower levels along with an extra isoform (#5). #5 isoform is detectable in normal hearts at later developmental stages (results not shown). The top right corner represents an overexposed blot B . The figure was adapted from Zhang et al. .