Figure 8

Markers of fibrosis suggest more pronounced injury in muscle from alcohol-fed mice. (A) Fibrotic index, a marker of collagen content quantified following trichrome staining, was increased at 7 and 14 days following injury in control-fed mice. Muscle from alcohol-fed mice reported a more robust fibrotic response at these same time points following injury. (B) Collagen VI, a component of fibrotic plaques, was increased 14 days after injury in muscle from alcohol-fed mice. (C) Fibronectin, an extracellular matrix glycoprotein associated with wound healing, was elevated in injured control muscle 14 days after injury. However, fibronectin expression was further increased in injured muscle from alcohol-fed mice. Significance was accepted at p ≤ 0.05. *, compared to control-fed, uninjured group. #, compared to control-fed, injured group matched to recovery time.