Fig. 5

Islet endothelial cell migration in cultured human islets in the absence of MSC. a In situ CD31 expression (green) two days after isolation showing vessels that are evenly spread throughout the islet. b After four days in culture after isolation, the vessels are withdrawn towards the center of the islet and after (c) one week in culture, the vessels are loosing their expression of CD31. d-f Sectioned human islets stained for CD31 (green) at 6 days after isolation. Nuclei stained by DAPI in blue. Bars = 100 um